Carbon monoxide prevents inner membrane permeabilization. Citrate synthase activity assay was used to follow inner membrane permeabilization. Measurements were performed at 412 nm in the absence or presence of 10 μM of CO and 5 μM of Ca2+ or 300 μM of atractyloside, at 37°C for 20 minutes. Representative graphics of experiments, which were done in triplicates and repeated, at least, three times are shown.
Queiroga et al. BMC Cell Biology 2011 12:10 doi:10.1186/1471-2121-12-10