Reducing the level of hRev7 in human fibroblasts renders them more sensitive to the cytotoxic effect of BPDE, but does not affect their frequency of BPDE-induced mutations. The parental cells strain (open circles); cell strains with reduced hRev7, designated clones 2-2 and 2-6 (closed symbols); the vector control strain (open triangles); and a cell strain in which to level of hRev7 was reconstituted, designated 2-2 + R7 (open squares); were treated with BPDE and assayed for (A) cell survival or (B) the frequency of mutations induced in the HPRT gene. The lines represent the least squares regression for the data.
Neal et al. BMC Cell Biology 2010 11:97 doi:10.1186/1471-2121-11-97