Additional file 3.
Distinct emission spectra of lipid droplets and membrane pellets isolated from dhs-28 animals that were vital-labelled by Nile Red. (A) In stage L4 dhs-28 animals that were grown and stained on 10-cm NGM/OP50/Nile Red plates, LROs displayed Spec-1 emission spectrum (pseudo-colored in red) and lipid droplets displayed Spec-2 (pseudo-colored in green). Similarly, membrane pellets (B) and lipid droplets (C) isolated biochemically from these dhs-28 animals displayed Spec-1 and Spec-2 respectively. (D) Emission spectrum profiles of Spec-1s (red line) and Spec-2s (green line) of intact dhs-28 animals and Spec-1s (dashed red line) and Spec-2s (dashed green line) of isolated membrane pellets and lipid droplets. Intensity values were normalized.
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Zhang et al. BMC Cell Biology 2010 11:96 doi:10.1186/1471-2121-11-96