Impact of histological stain and fixative/protector on morphology of goat mammary tissue and RNA integrity. Frozen mammary tissue sections were stained either with HistoGene® (A to C) or with Cresyl violet® (D and E). MEC isolated after treatment with RCL2, a new fixative preserving tissue morphology and Nucleic Acid integrity (C and D), or with RNA later (B) provide RNA of better quality, expressed as ΔRIN which is difference between the RIN value obtained with total RNA extracted from the mammary tissue section (8.5) and the RIN value obtained with RNA extracted from microdissected cells.
Bevilacqua et al. BMC Cell Biology 2010 11:95 doi:10.1186/1471-2121-11-95