Table 2

Validation of selected genes by quantitative real time PCR

Pathways

Genes

Microarray

QPCR

NA16

Colony

Tumor

NA16

Colony

Tumor


Hypoxia

Hif1a

NC

4.71

2.08

0.51

1.60

1.78

Stc1

55.90

NC

55.60

873.10

1.01

347.26

Vegfa

-2.5

3.73

NC

10.56

1.37

6.59

Vhl

3.68

NC

NC

6.45

1.12

1.80

Ddit3

8.45

2.14

4.64

11.31

1.52

1.00

Egln3

7.72

12.18

11.58

68.12

22.16

25.46


Glycolysis/Gluconeogenesis

Pfkm

3.60

-3.8

NC

4.29

1.32

2.35

HK2

4.78

NC

NC

29.02

1.36

3.05

Pdk1

9.11

NC

-2.75

13.00

1.00

2.00

Adh1

10.76

66.9

47.76

4.44

216.77

135.30

Aldh3a1

10.52

3.49

-2.36

3.36

18.38

1.04

Slc2a1

5.80

NC

2.11

27.86

1.64

1.78


Tumor formation/

metastasis

Spp1

NC

22.24

145.20

6.19

64.00

139.10

Mmp3

NC

33.52

87.30

0.03

0.04

6.68

Egfr

2.43

-2.46

NC

0.01

0.01

0.10

Rb1

2.48

3.17

3.60

0.69

1.12

2.64


Total RNA was collected from F111-NA16, Colony and tumour cells and validated for the selected genes of Hypoxia, glycolysis/gluconeogenesis and tumour formation/metastatsis from microarray data and were compared with control[F111-A16]. Genes showed the same trend in regulation in the qPCR and microarray experiments, but not necessarily by the same fold change. No change (NC) refers to a gene whose expression was below twofold with p Value less than 0.05 for the microarray.

Jinka et al. BMC Cell Biology 2010 11:93   doi:10.1186/1471-2121-11-93

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