Table 2

Selected genes downregulated by elevated cAMP/TPK3 signalling

Function

Gene

Fold Change

Gene Function


PKA

TPK1

-1.1

PKA catalytic subunit

TPK2

1.1

PKA catalytic subunit


ETS

NDE1

-2.0

Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH;

NDE2

-5.5

Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH;

SDH2

-2.3

Iron-sulfur protein subunit of succinate dehydrogenase Complex II

COQ2

-2.0

Catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis

RIP1

-1.7

Rieske Iron Sulphur Protein of Complex III

CYT1

-2.0

Cytochrome c1, catalytic subunit of complex III

CYT2

-2.3

Cytochrome c1 heme lyase, involved in maturation of CYT1

CYC1

-1.9

Cytochrome c, isoform 1;transfers electrons from Complex III to Complex IV

COX4

-2.2

Subunit IV of cytochrome c oxidase (Complex IV)

COX10

-2.3

Heme A:farnesyltransferase, required for cytochrome c oxidase activity;

SCO1

-2.0

Copper-binding protein of the mitochondrial inner membrane, required for cytochrome c oxidase

NCA3

-6.2

Regulates mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of Complex V

ATP19

-2.2

Subunit k of the mitochondrial F1F0 ATP synthase


Detox

SOD1

-1.4

Cytosolic superoxide dismutase

SOD2

-1.1

Mitochondrial superoxide dismutase; protects cells against oxygen toxicity

CTT1

-1.6

Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide


Selected genes involved in ETS and ROS detoxification that were downregulated in Δpde2 cells but not in Δpde2 Δtpk3 when grown in the presence of exogenous 4 mM cAMP. Gene lists were obtained as a ratio of statistically significant expression levels in Δpde2/Δpde2 Δtpk3 cells when grown in the presence of 4 mM cAMP.

Leadsham and Gourlay BMC Cell Biology 2010 11:92   doi:10.1186/1471-2121-11-92

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