HAP4 overexpression leads to the production of dysfunctional mitochondria prone to ROS production. Mitochondrial morphology was examined in wild type + empty plasmid, Δpde2 + empty plasmid and Δpde2 + HAP4 cells grown to diauxic shift in minimal media supplemented with 4 mM cAMP using a mitochondria targeted GFP probe (A). The expression of nuclear and mitochondria encoded components of the Cytochrome c Oxidase complex (complex IV) was examined in wild type and Δpde2 cells grown in the presence of 4 mM cAMP (B). The influence of HAP4 overexpression on the ability of wild type and Δpde2 cells to respire (C) and to produce ROS (D) was examined under the same growth conditions. Scale Bar = 10 μM.
Leadsham and Gourlay BMC Cell Biology 2010 11:92 doi:10.1186/1471-2121-11-92