Influence of transcription factors predicted to play a role in TPK3 based transcriptional remodeling under conditions of elevated cAMP. Cells lacking PDE2 and either of the transcription factors SKO1, SOK2 or HAP4 were examined for their capacity to regulate routine respiration, ROS production and cell death in response to cAMP addition. Routine respiration was measured in Δpde2, Δpde2Δsko1, Δpde2Δsok2, Δpde2Δhap4 cells using a high resolution respirometer after growth for 24 h in YPD + 4 mM cAMP (A). The same strains and growth conditions were used to assess the production of ROS using H2DCF-DA (B) and the extent of cell death within colonies using phloxine B uptake as described in materials and methods (C).
Leadsham and Gourlay BMC Cell Biology 2010 11:92 doi:10.1186/1471-2121-11-92