Elevated Tpk3p activity affects colony morphology. Cell death was visualized within growing yeast colonies grown for 5 days on rich media containing 10 μM Phloxine B containing 4 mM cAMP. Cells that are dead or dying accumulate the dye which stains them pink/red. Colonies were visualised from above before dissection and images captured incross section.
Leadsham and Gourlay BMC Cell Biology 2010 11:92 doi:10.1186/1471-2121-11-92