IFNgamma levels in culture supernatants were measured by ELISA. Culture supernatants were harvested from the same cell cultures used for cytotoxicity assays. (A) No antibody, (B) isotype-matched mAb, (C) blocking MICA mAb. IFNgamma measurements were made in triplicate from three separate assays. Values are shown as means ± SD from three independent experiments. *indicates a significant difference compared with the control group (p < 0.05).
Luo et al. BMC Cell Biology 2010 11:91 doi:10.1186/1471-2121-11-91