Transduction of HIF-1alpha siRNA can inhibit surface expression of MICA on HK-2 cells during hypoxia/reoxygenation. The relative protein expression levels were determined by flow cytometry with a PE conjugated mouse anti-human MICA mAb 8 h after reoxygenation. The percentage of cells stained by MICA mAb is shown by the numbers in the top right of each graph. (A) HK-2 cultured in hypoxia (B) HK-2 treated with negative control siRNA, cultured in hypoxia (C) HK-2 treated with HIF-1alpha siRNA, cultured in hypoxia (D) HK-2 cultured in normoxia, no treatment (E) Isotype control.
Luo et al. BMC Cell Biology 2010 11:91 doi:10.1186/1471-2121-11-91