Adenoviral mediated overexpression of HIF-1alpha and HIF-1alphaDELTAODD can up-regulate the surface expression of MICA on HK-2 cells in normoxia. The relative protein expression levels were determined by flow cytometry with a PE conjugated mouse anti-human MICA mAb 96 h after transduction in normoxia. The percentage of cells stained by MICA mAb is shown by the numbers in the top right of each graph. (A) HK-2 transduced with Ad.CMV.HIF-1alphaDELTAODD (B) HK-2 transduced with Ad.CMV.HIF-1alpha (C) HK-2 transduced with Ad.CMV.LacZ (D) HK-2 without transduction (E) Isotype control.
Luo et al. BMC Cell Biology 2010 11:91 doi:10.1186/1471-2121-11-91