Adenoviral mediated over-expression of HIF-1alpha and HIF-1alphaDELTAODD can up-regulate the transcription of VEGF and HO-1 in HK-2 cells in normoxia. The relative mRNA levels of HIF-1alpha, HO-1 and VEGF were determined by real time PCR and normalized to the housekeeping gene β-actin. (A) Relative mRNA levels of HIF-1alpha. (B) Relative mRNA levels of HO-1. (C) Relative mRNA levels of VEGF. Values are shown as means ± SD from three independent experiments. *indicates a significant difference compared with control group (p < 0.05).
Luo et al. BMC Cell Biology 2010 11:91 doi:10.1186/1471-2121-11-91