Additional file 5.

eGFP-Atg5 and Atg8b-GFP were expressed by GMR-GAL4 in imaginal discs. (A) Merged image of GFP expression pattern and Höechst 33342 stained pattern in eye imaginal disc of a GMR-GAL4;UAS-eGFP-Atg5. (B) eGFP-Atg5 expression pattern in eye imaginal disc of a GMR-GAL4;UAS-eGFP-Atg5. (B, C and D) Higher-magnification images of (A). (C) LysoTracker Red spots. (D) LysoTracker Red spots and eGFP-Atg5 spots were overlapped (white arrows indicate autophagosome). (E and F) Merged image of GFP expression pattern and Höechst 33342 stained pattern in eye imaginal disc of Hs-Atg8b-GFP larva after heat shock. (12 hour after five 1-hour heat shocks).

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Kim et al. BMC Cell Biology 2010 11:9   doi:10.1186/1471-2121-11-9