Additional file 4.
Expression level of Tango5, down-stream gene of Atg8a, was not affected by EP inserted up-stream of Atg8a. (A) The surrounding genes of Atg8a. CG1826 and CG15221 are located up-stream site of Atg8a. Tango5 is located down-stream of Atg8a and EP (G9749). (Black arrow indicates P-element insertion site of Atg8aEP(G9749)). (B) Genomic DNA of w1118 was used as a template for the lane 1, while appropriate cDNAs were used for the lanes 2, 3, 4 and 5. The lane 1 indicates that all the primer sets worked properly. The lane 2 indicates that Atg8a and Tango5 were expressed in the w1118 as the baseline level. The lane 3 indicates that Atg8a was up-regulated by the expression of Dcp-1. As showed in the lane 4 and 5,Atg8a expression level was increased in GMR-GAL4/Atg8aEP(G9749) and GMR-GAL4, Dcp-1/Atg8aEP(G9749). The level of Tango5 expression was detected slightly in the Lane 2, 3 and 4. However, Tango5 expression level was not increased by Atg8aEP(G9749).
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Kim et al. BMC Cell Biology 2010 11:9 doi:10.1186/1471-2121-11-9