Additional file 3.
Expression level of CG10969, down-stream gene of Atg1, was not affected by EP inserted up-stream of Atg1. (A) The surrounding genes of Atg1. CG10969, CG17666, CG17667 and CG11006 are located next to the Atg1. However, the directions of CG17666, CG17667 and CG11006 are opposite to the direction of EP (G13748) insertion. CG10969 is placed in down-stream site of Atg1 and EP (G13748). (Black arrow indicates the P-element insertion site of Atg1EP(G13748).). (B) The genomic DNA of w1118 was used as a template for the lane 1, while appropriate cDNAs were used for the lanes 2, 3, 4 and 5. The lane 1 indicates that all the primer sets worked properly. The lane 2 indicates that Atg1 and CG10969 were not expressed in the w1118. The lane 3 indicates that Atg1 was slightly up-regulated by the expression of Dcp-1. The lane 4 and 5 indicates that Atg1 expression level was increased in GMR-GAL4/Atg1EP(G13748) and GMR-GAL4, Dcp-1/Atg1EP(G13748). No expression was detected by the CG10969 primer set.
Format: TIFF Size: 723KB Download file
Kim et al. BMC Cell Biology 2010 11:9 doi:10.1186/1471-2121-11-9