Email updates

Keep up to date with the latest news and content from BMC Cell Biology and BioMed Central.

Open Access Research article

The effects of low frequency electrical stimulation on satellite cell activity in rat skeletal muscle during hindlimb suspension

Bao-Ting Zhang1, Simon S Yeung1, Yue Liu2, Hong-Hui Wang2, Yu-Min Wan2, Shu-Kuan Ling2, Hong-Yu Zhang2, Ying-Hui Li2* and Ella W Yeung1*

Author affiliations

1 Muscle Physiology Laboratory, Department of Rehabilitation Sciences, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong

2 Space Cell and Molecular Biology Laboratory, Astronaut Research and Training Centre of China, Beijing, PRChina

For all author emails, please log on.

Citation and License

BMC Cell Biology 2010, 11:87  doi:10.1186/1471-2121-11-87

Published: 18 November 2010

Abstract

Background

The ability of skeletal muscle to grow and regenerate is dependent on resident stem cells called satellite cells. It has been shown that chronic hindlimb unloading downregulates the satellite cell activity. This study investigated the role of low-frequency electrical stimulation on satellite cell activity during a 28 d hindlimb suspension in rats.

Results

Mechanical unloading resulted in a 44% reduction in the myofiber cross-sectional area as well as a 29% and 34% reduction in the number of myonuclei and myonuclear domains, respectively, in the soleus muscles (P < 0.001 vs the weight-bearing control). The number of quiescent (M-cadherin+), proliferating (BrdU+ and myoD+), and differentiated (myogenin+) satellite cells was also reduced by 48-57% compared to the weight-bearing animals (P < 0.01 for all). Daily application of electrical stimulation (2 × 3 h at a 20 Hz frequency) partially attenuated the reduction of the fiber cross-sectional area, satellite cell activity, and myonuclear domain (P < 0.05 for all). Extensor digitorum longus muscles were not significantly altered by hindlimb unloading.

Conclusion

This study shows that electrical stimulation partially attenuated the decrease in muscle size and satellite cells during hindlimb unloading. The causal relationship between satellite cell activation and electrical stimulation remain to be established.