Table 4

U937 cells' functional status after the freezing - thawing cycle.

Parameters measured (au)

Cell status and subgroups


Live cells (62%)

Late apoptosis (18%)

Early apoptotic cells


Total (19%)

MMP (+) (5%)

MMP (-) (14%)


TMRM mean FI

1476 ± 331

Negative

242.7 ± 155.6

429 ± 131

Negative


TMRM FI SD

652 ± 249

Negative

97.2 ± 11.2

141 ± 47

Negative


FDA slop

36.1 ± 11.36

Negative

9.6 ± 11.2

11 ± 7.1

9.05 ± 12.2


U937 cells underwent a freezing - thawing cycle as described in Materials and Methods. Overnight after thawing the multi-parametric analysis was performed and four parameters were elicited for each single cell. The cell subgroups were defined as described in Multi-parametric analysis following the freezing - thawing cycle in the i3C section. Values are average ± SD in individual cells' subgroups. The percentage of each subgroup from the total population in a representative experiment is shown in brackets.

Afrimzon et al. BMC Cell Biology 2010 11:83   doi:10.1186/1471-2121-11-83

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