Fluorescein diacetate hydrolysis rates at 19 hours after thawing. For the evaluation of the metabolic recovery after the freezing - thawing cycle, the cells were stained by FDA and the linear slope FI(t) was calculated for each single cell by image analysis. The mean slopes for U937 cell groups that underwent the freezing - thawing cycle in the cryo-devices (at least 4 experiments for each cryo-device) are presented as mean ± SD.
Afrimzon et al. BMC Cell Biology 2010 11:83 doi:10.1186/1471-2121-11-83