Monitoring U937 cell membrane integrity after the freezing - thawing cycle by FDA staining. The U937 cells were exposed to the freezing - thawing cycle in the i3C. At 3 hours after thawing, FDA solution (1.2 μM) was introduced into the i3C and images were taken 5 minutes after FDA addition. The transmitted image (A) and corresponding fluorescent image (B) are presented. The dead U937 cell unstained by FDA is indicated by the red arrow. Scale bar: 0.01 mm.
Afrimzon et al. BMC Cell Biology 2010 11:83 doi:10.1186/1471-2121-11-83