Figure 7.

PIST is expressed in P5 mouse inner ear. (A) Mouse organ of Corti immunolabeled with rabbit antibodies to PIST (detected with FITC-conjugated secondary antibodies, green) and with guinea pig antibodies to myosin VIIa (Myo7a, detected with Cy5-conjugated secondary antibodies, red). (B) Mouse organ of Corti at higher magnification reveals PIST immunoreactivity in organ of Corti supporting cells, outer hair cells (OHC), and albeit weaker, in inner hair cells (IHC). The extracellular tectorial membrane (TM) shows unspecific labeling with the PIST antibody. (C) Mouse utricle and saccule staining. (D) Mouse utricle staining at higher magnification. (E) Mouse ampulla staining. DAPI (blue) was used to label cell nuclei. TRITC-conjugated Phallodin (white) was used to label stereocilia. Scale bars, 50 μm.