Co-localization of cadherin 23 with PIST in transfected HEK293 cells. A. Myc-tagged cadherin 23 localizes in the cytoplasm. B. In the presence of EGFP-tagged PIST, Myc-tagged cadherin 23 co-localizes with EGFP-PIST at TGN-like structures. C. Myc-tagged cadherin 23 without the last 4 amino acids (ITEL) localizes in the cytoplasm. D. In the presence of EGFP-PIST, Myc-tagged cadherin 23 without the last 4 amino acids (ITEL) remains in the cytoplasm, albeit EGFP-PIST localizes at TGN-like structures. Myc-cadherin 23 was stained with mouse anti-Myc antibody and then TRITC-conjugated goat anti-mouse secondary antibody. Nuclei were stained with TOTO-3. Scale bars, 10 μm.
Xu et al. BMC Cell Biology 2010 11:80 doi:10.1186/1471-2121-11-80