Interaction between PIST and cadherin 23. A. Schematic diagram of chicken cadherin 23 domain structure and the intracellular fragment used as the bait for yeast two-hybrid screens. B. Schematic diagram of chicken PIST domain structure and PIST protein fragments encoded by the clones isolated from yeast two-hybrid screens. C. Western blots showing co-immunoprecipitation (co-IP) of EGFP-tagged human PIST protein with Myc-tagged mouse cadherin 23(+68) and cadherin 23(-68). The co-IP is almost undetectable when the last 4 amino acids (ITEL) of cadherin 23 are deleted. IP indicates antibody used for immunoprecipitation and WB indicates antibody used for detection.
Xu et al. BMC Cell Biology 2010 11:80 doi:10.1186/1471-2121-11-80