Characteristics of hAFS cells obtained by the starter cell method. (A) Clonal hAFS cells at passage 18 show an Oct-4 specific signal after staining with FITC-conjugated secondary antibody against anti-Oct4a with fluorescent microscope at 20× magnification. The Oct-4 positive signal is exhibit in hAFS population as shown by merge of Oct-4a and Hoechst 33342 staining. (B) The hAFS cell line at passage 18 has expression of Oct-4a and HLA-ABC, but not HLA-DR, Nanog and Sox2 by cDNA analysis using RT-PCR. The expression of Nestin was observed in hAFS cell-derived neurons. C means negative control, while gDNA was used as positive control of PCR amplification. (C) The flow cytometry analysis shows expression of SSEA4, CD29, CD44, CD73, CD90, CD105 and CD133, but not CD34 or CD45 on the hAFS cell surface.
Phermthai et al. BMC Cell Biology 2010 11:79 doi:10.1186/1471-2121-11-79