Figure 4.

Phosphorylation of ERK1/2 but not AKT in ISCs is upregulated by GDNF. (A) Time-course of ERK1/2 phosphorylation levels in cultured ISCs stimulated with GDNF and the first bar (0) is cells without GDNF treatment. Total ERK1/2 was the internal control in the semi-quantitative densitometry analysis (right panel). (B) Time course of AKT phosphorylation levels in ISCs stimulated with GDNF, with total AKT as an internal control in the semi-quantitative densitometry analysis (right panel). (C) Time course of ERK1/2 phosphorylation level in PD98059 pre-treated (10 μM) ISCs stimulated with GDNF and the bar (control) is cells without GDNF or PD98059 treatment, with total ERK1/2 as the internal control in the semi-quantitative densitometry analysis (right panel). (D) Time course of AKT phosphorylation levels in PD98059 pre-treated (10 μM) ISCs stimulated with GDNF, with total AKT as the internal control in the semi-quantitative densitometry analysis (right panel). The data were presented as means ± SD from three independent experiments. Statistically significant differences (p < 0.05) among groups are indicated by an asterisk.

Yang and Han BMC Cell Biology 2010 11:78   doi:10.1186/1471-2121-11-78
Download authors' original image