RNase protective assay of α-, ε-, γ- and β-globin gene mRNA expression in K562 cells before and after erythroid differentiation. (A) Globin gene expression in un-induced K562 cells. (B) Globin gene expression in K562 cells 72 h after treatment with hemin. K562 and pc3.1 represent K562 cells and stable K562/pc3.1 transfectant pool respectively. A, B, C, D and E represent the five stable K562/pc3.1-CTDSPL2 transfectant pools respectively.
Ma et al. BMC Cell Biology 2010 11:75 doi:10.1186/1471-2121-11-75