Effects of cell transplantation on blood flow recovery in the ischemic hind limb. A) Strategy to purify Flk-1+ cells and to transplant them into ischemic hind limb tissues. B) Representative LDBF images. A low perfusion signal (dark blue) was observed in the ischemic left hind limb of control mice, whereas high perfusion signals (white to red) were detected in Flk-1+ cell-transplanted animals (6 × 104 cells) on postoperative days 3, 7 and 14. C) Quantitative analysis of the ischemic/nonischemic limb LDBF ratio on pre (Day-1) and post operative days 0, 3, 7 and 14 (n = 4). *p < 0.05 Flk1+ cells (6×104) injected mice vs. control mice. D) Cell dose-dependent effect of transplantation seven days after surgery. Flk-1+ cells (2 × 103, 2 × 104 or 6 × 104 cells) or PBS as a control were injected into the ischemic limb at postoperative day 1 (n = 4/each group, **p < 0.05 2×104 or 6×104 vs. control mice).
Suzuki et al. BMC Cell Biology 2010 11:72 doi:10.1186/1471-2121-11-72