Time course RT-CPR in vitro. The expression of Flk-1 was peaked at Day5 from differentiation. Undifferentiated iPS cells markers, Nanog and Oct3/4, were strongly expressed in early phase and started to gradually decrease with differentiation. We also observed the transient expression of c-myc at Day4 and Day5.
Suzuki et al. BMC Cell Biology 2010 11:72 doi:10.1186/1471-2121-11-72