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Spindle assembly checkpoint genes reveal distinct as well as overlapping expression that implicates MDF-2/Mad2 in postembryonic seam cell proliferation in Caenorhabditis elegans

Maja Tarailo-Graovac, Jun Wang, Jeffrey SC Chu, Domena Tu, David L Baillie and Nansheng Chen*

Author Affiliations

Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada

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BMC Cell Biology 2010, 11:71  doi:10.1186/1471-2121-11-71

Published: 21 September 2010

Additional files

Additional file 1:

Figure S1: Putative mdf-1/MAD1 promoter activities. (A) 295 bp of the 5' regulatory region immediately upstream of his-35, the first gene in the mdf-1 containing operon, drives localized GFP expression from the embryonic stage. (Left) GFP images; (Right) DIC images. (B) The internal promoter is the 543 bp sequence between his-35 and mdf-1. This promoter drives ubiquitous GFP expression in the embryo as expected. (C) 1332 bp of the 5' regulatory region upstream from the ATG initiator site in mdf-1 - extending to the operon adjacent upstream gene (his-41) - results in ubiquitous GFP expression in embryos, similar to the internal mdf-1 promoter expression pattern.

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