Email updates

Keep up to date with the latest news and content from BMC Cell Biology and BioMed Central.

Open Access Research article

Knockdown and overexpression of Unc-45b result in defective myofibril organization in skeletal muscles of zebrafish embryos

Elena P Bernick12, Pei-Jun Zhang23 and Shaojun Du12*

Author Affiliations

1 University of Maryland School of Medicine Interdisciplinary Training Program in Muscle Biology, Baltimore, MD 21201, USA

2 Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA

3 Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China

For all author emails, please log on.

BMC Cell Biology 2010, 11:70  doi:10.1186/1471-2121-11-70

Published: 17 September 2010

Additional files

Additional file 1:

Figure 1 (supplement) showed that overexpression of EGFP or Hsp90a1 had not effect on thick filament organization. A. DNA constructs expressing EGFP or myc-tagged Hsp90a1 in skeletal muscles of zebrafish embryos were directed using the smyd1 promoter. Myosin thick filament organization was analyzed by F59 staining. B-D. Single staining with anti-myosin (F59) antibody (red) shows that expression of GFP (B) has no effect on myosin thick filament organization (C, D). E-G. Double staining shows the expression of myc-tagged Hsp90a1 (E) in a single fiber and its lack of effect on myosin thick filament organization (F, G). G represents the merged image of E and F. Scale bars = 20 mm.

Format: PPT Size: 4MB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Open Data