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Open Access Highly Accessed Methodology article

Intracellular localization and interaction of mRNA binding proteins as detected by FRET

Pamela S David Gerecht1, Molly A Taylor2 and J David Port13*

Author Affiliations

1 Departments of Medicine/Cardiology and Pharmacology, University of Colorado School of Medicine, 12700 East 19th Avenue, Aurora, CO 80045, USA

2 Department of Pharmacology, Case Western Reserve University, 2103 Cornell Road, Cleveland, OH 44106, USA

3 University of Colorado School of Medicine Division of Cardiology, B139 12700 East 19th Avenue, Rm 8001 Aurora, CO 80045, USA

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BMC Cell Biology 2010, 11:69  doi:10.1186/1471-2121-11-69

Published: 15 September 2010

Additional files

Additional file 1:

Video 1. Interactions of PBs and SGs in DDT1-MF2 cells subjected to oxidative stress. DDT1-MF2 cells were treated with 0.5 mM sodium arsenite for 30 minutes prior to immunostaining. PBs were detected by Hedls (red) and SGs by KSRP (green). Images were collected along the Z axis of the cell at 100× magnification under oil immersion, (lens numerical aperture 1.40), using 2 × 2 binning mode on an inverted Nikon Eclipse TE3000 fluorescence microscope using a Cooke SensiCam QE CCD camera running Slidebook software (3I, version 4.0.1.43). Capture time was 500 ms and step size was 0.5 microns. 3D stack was "deconvolved" using the nearest-neighbors method.

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