Additional file 4.
cAMP-induced expression of CLDN5 and OCLN is mediated by PKA. (A) HUVEC were pretreated with H-89 for 30 minutes and stimulated with cAMP or its derivates for up to 72 h. mRNA expression of OCLN and CLDN5 were determined by real-time qPCR and normalized to expression of HPRT-1 (n = 3). (B) Shown are representative western blot images of HUVEC pretreated with H-89 for 30 minutes and stimulated with cAMP derivates for up to 72 h. Protein amounts of tubulin served as loading control (n = 2).
Format: EPS Size: 3.5MB Download file
Beese et al. BMC Cell Biology 2010 11:68 doi:10.1186/1471-2121-11-68