Long-term effect of cAMP and its derivates on formation of tight junctions. HUVEC were stimulated for 72 h with the different cAMP derivates, stained for tight junction-associated proteins ZO-1, OCLN, CLDN5 and Jam-A and analyzed by confocal laser microscopy. The nucleus was counterstained with DAPI. Shown are representative micrographs of at least three independent experiments using HUVEC from different donors. Scale bar represents 25 μm.
Beese et al. BMC Cell Biology 2010 11:68 doi:10.1186/1471-2121-11-68