Figure 5.

Molecular mechanisms involved in hypoxia-induced MIF up-regulation. Cells were pretreated with ERK inhibitor (PD98059, 10-6 mol/L), or anti-oxidant (Tiron, 5 mmol/L) and then exposed to hypoxia (3% O2) for 24 hours. In some experiments, cells were transfected with MIF-siRNA for 24 h and then exposed to normoxia or hypoxia (3% O2) for 24 h. (A) Real-time PCR results. MIF mRNA expression were assayed by Q-PCR (n = 3 in each group). * indicates P < 0.05 vs control cells under normoxia. # P < 0.05 vs control cells under hypoxia. (B) ELISA results. MIF protein released into cell culture media was measured by ELISA (n = 3 in each group). * indicates P < 0.05 vs control cells under normoxia. # P < 0.05 vs control cells under hypoxia. (C) Western blot results. Representative Western blot (top) and values of total MIF production (mean ± SEM of 3 experiments, bottom). Results of total MIF protein production were obtained from densitometric analysis and expressed as ratio of MIF/β-actin. * indicates P < 0.05 vs control cells under normoxia. # P < 0.05 vs control cells under hypoxia.

Fu et al. BMC Cell Biology 2010 11:66   doi:10.1186/1471-2121-11-66
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