Differential expression of HMGA1 proteins during C2C12 cell differentiation. (A) Down-regulation of HMGA1 during myogenic differentiation of C2C12 cells as analyzed by PCR and Western blotting (WB). For Western blots proteins of 1.5 × 105 nuclei were separated on a 15% SDS-polyacrylamide gel. For RT-PCR 1 μg of total RNA was used to produce cDNAs for the PCR reaction. For PCR identical amounts of cDNA were used. Time points of analyses are indicated in days. The arrowhead marks the time point of induction. Day 0 denotes non-induced myoblasts. Gapdh expression served as a control for the reverse transcription in RT-PCR experiments. As a control for loading and Western blotting Ponceau staining (P) of core histones is shown. (B) Down-regulation of HMGA1 expression during osteogenesis. Osteogenesis in C2C12 cells was induced with 0.5 μg/ml BMP2 (arrowhead) and HMGA1 expression was analyzed at day 0 and on days 1, 3 and 4 after by RT-PCR (RT) and Western blot (WB) as described in (A).
Brocher et al. BMC Cell Biology 2010 11:64 doi:10.1186/1471-2121-11-64