Figure 6.

KPNA7 localizes to the nucleus in HeLa cells. (A) Immunofluorescence microscopy of endogenous KPNA2 and KPNA7 in HeLa cells shows that KPNA7 is nuclear at steady state. (B) HA-KPNA2 and HA-KPNA7 transfected into HeLa cells, and detected with an anti-HA antibody by immunofluorescence microscopy. (C) Immunoblot of HeLa lysates probed with purified anti-KPNA7, or anti-KPNA7 in the presence of excess KPNA7 peptide to show competition for antibody binding.