Monopartite and bipartite NLS binding of the importin α family. Binding reactions between in vitro translated 35 S methionine labeled human KPNA's in solution and (A) GST-SV40 NLS (monopartite) and GST-RB NLS (bipartite) immobilized upon glutathione agarose beads, or (B) GST-NPM NLS (NPM 150-175). As a negative control, GST was used alone.
Kelley et al. BMC Cell Biology 2010 11:63 doi:10.1186/1471-2121-11-63