Additional file 5.

Dominant-negative SNARE domains inhibit trafficking of transferrin into a perinuclear compartment. CHO-K1 cells were transiently transfected for 22 h with truncated GFP-tagged constructs of VAMP3 (VAMP3-cyto) [A], syntaxin13 (syntaxin13-cyto) [C], VAMP4 (VAMP4-cyto) [G], or 15 h with SNAP23 (SNAP23^CΔ9) [E]. To load the perinuclear recycling compartment with Tfn, cells were serum starved for 1 hr followed by incubation with 30 μg/mL rhodamine-Tfn in serum-free media for 15 min. Coverslips were briefly washed with 0.2 M glycine, pH 2.5, to remove surface bound Tfn, washed three times with PBS and fixed in 4% paraformaldehyde in PBS. Arrows in B, D and F point to perinuclear, rhodamine-Tfn-containing compartment in non-transfected control cells. Images are 3 D reconstructions of a z-series. Scale bar represents 10 μm

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Skalski et al. BMC Cell Biology 2010 11:62   doi:10.1186/1471-2121-11-62