Additional file 3.

The Golgi apparatus, labeled by antibodies to Golgin subfamily A member 5 (GOLGA5), in 6 d wild-type and Crb2a transgenics. (A-G"') Confocal z-projection of GFP-expressing rods in sections of 6 d retinas labeled with anti-GOLGA5 antibodies (red) and anti-HA (blue). (A, A') Wild-type rods. (B-B"') Crb2aFL transgenic rods. (C-C"') Crb2aExtra_TM transgenic rods. (D-D"') Crb2aExtra_Secr transgenic rods. (E-E"') Crb2aIntraWT transgenic rods. (F-F"') Crb2aIntraΔFBD transgenic rods. (G-G"') Crb2aIntraΔPBD transgenic rods. (H) Western blot of 5 d wild-type zebrafish labeled with anti-GOLGA5 antibodies reveals a single protein of the expected molecular weight. The western blot was performed as described in [8], anti-GOLGA5 (Sigma HPA000992) was used at 1:1000 and HRP-conjugated goat anti-rabbit was used at 1:30,000. Scale bar, 5 μm.

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Hsu and Jensen BMC Cell Biology 2010 11:60   doi:10.1186/1471-2121-11-60