Figure 4.

Measurement of rod size in Crb2a transgenics. (A) The average combined volume of rod cell body and inner segment (black circles) measured 332 ± 30 μm3 (wildtype), 372 ± 13.6 μm3 (Crb2aIntraWT), 394 ± 12.2 μm3 (Crb2aIntraΔFBD), 416 ± 28.9 μm3 (Crb2aIntraΔPBD), and 436 ± 22.8 μm3 (Crb2aIntraΔFBDΔPBD). The average volume of a rod outer segment (red circles) measured 190 ± 15.7 μm3 (wildtypes), 277 ± 15.4 μm3 (Crb2aIntraWT), 283 ± 20.6 μm3 (Crb2aIntraΔFBD), 278 ± 15.9 μm3 (Crb2aIntraΔPBD), and 212 ± 18.9 μm3 (Crb2aIntraΔFBDΔPBD). (B) The average combined volume of rod cell body and inner segment (black circles) measured 332 ± 30 μm3 (wildtype), 426 ± 17.5 μm3 (Crb2aFL), 401 ± 17.8 μm3 (Crb2aExtra_TM), and 405 ± 11 μm3 (Crb2aExtra_Secr). The average volume of a rod outer segment (red circles) measured 190 ± 15.7 μm3 (wildtype), 198 ± 13.8 μm3 (Crb2aFL), 316 ± 17.2 μm3 (Crb2aExtra_TM), and 268 ± 12.1 μm3 (Crb2aExtra_Secr). (C, C') Width of the inner segment myoid region was marked as in (C) and the measurements are shown in (C'). The width of the myoid region is significantly larger in Crb2aFL rods (2.2 ± 0.1 μm) than wildtypes (1.5 ± 0.08 μm). Crb2aExtra_TM (1.5 ± 0.07 μm) and Crb2aExtra_Secr (1.6 ± 0.06 μm) are similar to wildtypes. (D, D') Area of the myoid region was outlined as in (D) and the measurements are shown in (D'). The area of the myoid region is significantly larger in Crb2aFL (10.7 ± 0.5 μm2), Crb2aExtra_TM (8.1 ± 0.4 μm2), and Crb2aExtra_Secr (9.1 ± 0.5 μm2) compared to wildtypes (6.7 ± 0.2 μm2). Each circle represents a measurement of a single rod. Shown values are mean ± s.e.m, *, p < 0.005, student's t test.

Hsu and Jensen BMC Cell Biology 2010 11:60   doi:10.1186/1471-2121-11-60
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