Expression of insulin 2(C96Y)-EGFP results in the induction of selected genes. Clone #4S2 cells were untreated (Control) or treated with 2 μg/ml doxycycline (+Dox) for the times indicated. Total RNA was isolated and the mRNA levels relative to cellular β-actin for Erdj4/Dnajb9 (A), Erdj5/Dnajc10 (B), Ero1β (C), Ero1α (D) were analyzed by real-time PCR as outlined in the methods. Shown are the mean ± SE of 3 independent experiments. *p < 0.05 relative to control.
Hartley et al. BMC Cell Biology 2010 11:59 doi:10.1186/1471-2121-11-59