Email updates

Keep up to date with the latest news and content from BMC Cell Biology and BioMed Central.

Open Access Highly Accessed Research article

Endoplasmic reticulum stress response in an INS-1 pancreatic β-cell line with inducible expression of a folding-deficient proinsulin

Taila Hartley12, Madura Siva13, Elida Lai12, Tracy Teodoro12, Liling Zhang1 and Allen Volchuk1234*

Author Affiliations

1 Division of Cellular and Molecular Biology, Toronto General Research Institute, University Health Network, 101 College Street, TMDT 10-706, Toronto, M5G 1L7, Canada

2 Department of Biochemistry, University of Toronto, 1 King's College Circle, Medical Sciences Building 5th Floor, Toronto, M5S 1A8, Canada

3 Department of Physiology, University of Toronto, 1 King's College Circle, Medical Sciences Building 3rd Floor, Toronto, M5S 1A8, Canada

4 University Health Network, 101 College Street, TMDT 10-706, Toronto, M5G 1L7, Canada

For all author emails, please log on.

BMC Cell Biology 2010, 11:59  doi:10.1186/1471-2121-11-59

Published: 26 July 2010

Additional files

Additional file 1:

This file lists all the genes whose expressed increased or decreased >2-fold in at least two of three independent experiments. Excel file presenting all of the Affymetrix probes whose expression was increased or decreased by at least 2 fold between doxycycline-treated vs. control, untreated INS-1 clone #4S2 cells. Data from 3 independent experiments (N = 1, N = 2, N = 3) for all doxycycline treatment time points (24 h, 48 h, and 5 days) is shown. Note that gene descriptions for these tables are provided by the GeneSpring software. Bold indicates probes whose expression was changed in all three experiments, while regular font indicates genes that were changed in at least 2 of 3 independent experiments. The primary data files for the microarray experiments have been deposited in the Gene Expression Omnibus (GEO) database (GSE22537).

Format: XLS Size: 62KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

This file lists the well substantiated genes whose expressed increased or decreased >2-fold in at least two of three independent experiments. Excel file presents expression data for well substantiated genes derived from the data presented in additional file 1. The putative function of the genes is derived from the NCBI database. Data from 3 independent experiments for all doxycycline treatment time points is provided.

Format: XLS Size: 55KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data