H2O2 provoked DNA-PK and JNK activity.(A) InR1-G9 cells were treated with Zeocin (50, 100 or 200 μg/ml) for indicated time before harvested. Oct-1 expression in the nuclear extract (NE) was then assessed. (B) InR1-G9 cells were treated with vehicle, Zeocin (100 μg/ml) or H2O2 (00 μM) for 2 h before harvested for DNA-PK assay. The values are mean +/- S.E (n = 3). *, P < 0.05. (C, D) InR1-G9 cells were treated with forskolin/IBMX (C) or H2O2 (100 μM) (D) for indicated time. Whole cell lysates were utilized for assessing the expression of JNK and phosphorylated JNK, ERK and phosphorylated ERK.
Wang and Jin BMC Cell Biology 2010 11:56 doi:10.1186/1471-2121-11-56