Validation of FMNL2 antibody. a) Western blot analysis of FMNL2 protein in seven human cell lines. A single band, of approximately 140 kDa, is detected in five cell lines. b) Western blot analysis with FMNL2 antibody competed either with GST-FMNL2 fusion protein or GST. Competition with GST-FMNL2 but not GST results in disappearance of the 140 kDa FMNL2 band. c) Western blot analysis of WM164 and MKN-1 cells transfected with FMNL2-specific siRNA, non-targeting (nt) siRNA or without siRNA (control). FMNL2 siRNA markedly reduces FMNL2 reactivity in both cell types. The α-tubulin blot confirms equal protein loading. d) Northern blot analysis of FMNL2 mRNA in six human cell lines. A 5.6 kb band is seen in all five cell lines, in which the Western blot analysis detects the protein band.
Gardberg et al. BMC Cell Biology 2010 11:55 doi:10.1186/1471-2121-11-55