Cell retention. Box plot of the percentage of dislodging cells during the freezing - thawing cycle. U937 cells were loaded into the i3C (n = 5), and the same area (20 × 20 picowells) was imaged in the course of cell handling. Total cells dislodging was calculated after each step: 1 - after pre-cooling, 2 - immediately after thawing, 3 - post-thawing after opening the cryostage lid, 4 - after post-thawing medium exchange. The mean value for each data set is indicated by the central line and the first and third quartiles are the edges of the box area.
Deutsch et al. BMC Cell Biology 2010 11:54 doi:10.1186/1471-2121-11-54