Expression of exogenous HER2 prevents the arecoline disruption of ZO-1 protein localization in Ishikawa cells. The CMV-HER2 and CMV-neo empty vector transfected Ishikawa cells were treated with the DMSO vehicle control or with 0.3 mM Arecoline for 24 hours and 48 hours. Cells were fixed in 3.5% formaldehyde and stained for the localization of ZO-1 by indirect immunofluorescence or for nuclear DNA by DAPI staining. Bars = 20 μm.
Giri et al. BMC Cell Biology 2010 11:53 doi:10.1186/1471-2121-11-53