Expression of exogenous HER2 prevents the arecolin down-regulation of ZO-1 expression in Ishikawa cells. (A) Ishikawa cells were stably transfected with either the CMV-HER2 expression plasmid or the CMV-neo empty vector control plasmids and transfection competent cells selected in media containing G418. Western blotting shows that the in CMV-HER2 transfected cells, HER2 protein levels are over-expressed compared to cells transfected with the CMV-neo empty vector. (B) CMV-HER2 transfected and CMV-neo transfected cells were treated with or without 0.3 mM arecoline for 48 hrs and the level of ZO-1 protein was determined by western blotting. The level of actin protein was used as a gel loading control.
Giri et al. BMC Cell Biology 2010 11:53 doi:10.1186/1471-2121-11-53