Arecoline down regulates the level of ZO-1 and HER2 proteins in both the Cytoplasmic/membrane as well as nuclear fractions of Ishikawa cells. The Ishikawa cells were treated with the DMSO vehicle control, 0.1 mM, 0.3 mM, or 0.5 mM arecoline for 48 hours. The nuclear and cytoplasmic/memebrane fraction was separated biochemically by differential centrifugation, and distribution of ZO-1 and HER2 was evaluated by western blot analysis in comparison to the lamin nuclear marker protein.
Giri et al. BMC Cell Biology 2010 11:53 doi:10.1186/1471-2121-11-53