Immunofluorescence analysis of the arecoline disruption of ZO-1 protein localization. Ishikawa cells were treated with the DMSO vehicle control, 0.1 mM, 0.3 mM, or 0.5 mM arecoline for 24 and 48 hrs. Cells were fixed in 3.7% formaldehyde and stained for localization of ZO-1 by indirect immunofluorescence and for nuclear DNA by DAPI staining. Bars = 20 μm.
Giri et al. BMC Cell Biology 2010 11:53 doi:10.1186/1471-2121-11-53