Effects of increasing concentrations of EGTA on ionomycin-induced production of superoxide anions by neutrophils. Neutrophils were stimulated with ionomycin (5 × 10-7 M final concentration) without or with increasing concentrations of the Ca2+ chelator EGTA. EGTA was added to the samples 20 seconds before the addition of the ionophore (time 0). Intracellular production of superoxide was assessed using a luminol-amplified chemiluminescence technique.
Light emission was measured with or without EGTA, and a representative experiment of at least five performed is shown. Abscissa, time of study (minutes); ordinate, superoxide production, given as light emission and expressed as cpm × 10-6.
Forsman and Dahlgren BMC Cell Biology 2010 11:52 doi:10.1186/1471-2121-11-52